Human VEGF (Research Grade Bevacizumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9947
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu101
Product Specifications
Immunogen
Human VEGF-A
Specificity
Detects human VEGF based on Bevacizumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Bevacizumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human VEGF (Research Grade Bevacizumab Biosimilar) Antibody
Cell Proliferation Induced by VEGF165and Neutralization by Human VEGF (Bevacizumab Biosimilar) Antibody.
Recombinant Human VEGF165(Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165(10 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human VEGF (Bevacizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9947). The ND50 is typically 10-80 ng/mL.Applications for Human VEGF (Research Grade Bevacizumab Biosimilar) Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-80 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF
Long Name
Vascular Endothelial Growth Factor
Alternate Names
MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF
Entrez Gene IDs
Gene Symbol
VEGFA
Additional VEGF Products
Product Documents for Human VEGF (Research Grade Bevacizumab Biosimilar) Antibody
Product Specific Notices for Human VEGF (Research Grade Bevacizumab Biosimilar) Antibody
For research use only
Loading...
Loading...
Loading...
Loading...