Human Versican Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3054
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 255915
Product Specifications
Immunogen
E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611
Gly1344-Asp1554
Accession # P13611
Specificity
Detects human Versican in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for Human Versican Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Versican, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Versican, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Versican
Sample: Recombinant Human Versican
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Versican
Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Alternate Names
CSPG2, GHAP, PG-M, VCAN
Gene Symbol
VCAN
UniProt
Additional Versican Products
Product Documents for Human Versican Antibody
Product Specific Notices for Human Versican Antibody
For research use only
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