Human VHL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11435
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1066402
Product Specifications
Immunogen
E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337
Asn150-Asp213
Accession # P40337
Specificity
Detects human VHL in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VHL Antibody
Detection of Human VHL by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cells, loaded at 0.2 mg/mL. A specific band was detected for VHL at approximately 23 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.
Detection of Human VHL by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB11435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for VHL at approximately 17, 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human VHL Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: K562 human chronic myelogenous leukemia cells
Sample: K562 human chronic myelogenous leukemia cells
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells
Sample: K562 human chronic myelogenous leukemia cells and Daudi human Burkitt's lymphoma cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VHL
References
- Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
- Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
- Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
- Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411
Long Name
Von Hippel-Lindau Tumor Suppressor
Alternate Names
Protein G7, pVHL
Gene Symbol
VHL
UniProt
Additional VHL Products
Product Documents for Human VHL Antibody
Product Specific Notices for Human VHL Antibody
For research use only
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