Human VIPR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5416
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 476031
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human VIP R2
Glu24-Ile438
Accession # P41587
Glu24-Ile438
Accession # P41587
Specificity
Detects human VIP R2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VIPR2 Antibody
VIP R2 in Human Brain.
VIP R2 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 25 µg/mL Mouse Anti-Human VIP R2 Monoclonal Antibody (Catalog # MAB5416) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human VIPR2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VIPR2
Human VIP R2 (Vasoactive intestinal polypeptide receptor 2) is a 47 kDa (predicted) seven transmembrane receptor that belongs to the G protein coupled receptor 2 family of proteins. In addition to the seven transmembrane regions, the mature chain also contains in its first extracellular region a hormone receptor domain (aa 49‑114), which includes three N-linked glycosylation sites. Human VIP R2 shares 85% aa identity with mouse and rat VIP R2. VIP R2 is expressed predominantly in skeletal muscle and to a lesser extent in the brain, heart, pancreas, and placenta. VIP R2 is coupled to a cAMP mediated signal transduction pathway and binds VIP and pituitary adenylate cyclase activating polypeptide (PACAP).
Long Name
Vasoactive Intestinal Peptide Receptor 2
Alternate Names
PACAPR3, VIP R2, VPAC2, VPCAP2R
Gene Symbol
VIPR2
UniProt
Additional VIPR2 Products
Product Documents for Human VIPR2 Antibody
Product Specific Notices for Human VIPR2 Antibody
For research use only
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