Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P) and either (A) Mouse Anti-Human VISTA/B7-H5/PD-1H APC-conjugated Monoclonal Antibody (Catalog # FAB71261A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human VISTA/B7-H5/PD-1H APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cell (PBMCs)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB71261A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

Q9H7M9 (Human)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Human VISTA/B7-H5/PD-1H APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human VISTA/B7-H5/PD-1H APC-conjugated Antibody

For research use only

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Reviews

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Flow Cytometry

Human VISTA/B7-H5/PD-1H APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB71261A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VISTA/B7-H5/PD-1H APC-conjugated Antibody

Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry.