Human VSIG10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9969

R&D Systems, part of Bio-Techne
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MAB9969-100
MAB9969-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 988622

View all VSIG10 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human VSIG10
Met1-Gly413
Accession # Q8N0Z9

Specificity

Detects human VSIG10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VSIG10 Antibody

VSIG10 antibody in RT-4 and MOLT-4 Human Cell Lines by Immunocytochemistry (ICC).

VSIG10 in RT-4 and MOLT-4 Human Cell Lines.

VSIG10 was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line (left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control; right panel) using Mouse Anti-Human VSIG10 Monoclonal Antibody (Catalog # MAB9969) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human VSIG10 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line and MOLT-4 human cute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG10

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Human VSIG10 cDNA encodes a 540 amino acid (aa) precursor that contains a 30 aa signal sequence, a 383 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 106 aa cytoplasmic domain. Human VSIG10 ECD contains four Ig-like C2-type domains and shares 95%, 69% and 71% aa identity with chimpanzee, mouse and rat VSIG10, respectively. VSIG10 is structurally related to the B7 family of immune regulatory proteins.

Long Name

V-Set and Immunoglobulin Domain Containing 10

Alternate Names

FLJ20674, V-set and immunoglobulin domain containing 10, V-set and immunoglobulin domain-containing protein 10

Entrez Gene IDs

54621 (Human); 231668 (Mouse); 304529 (Rat)

Gene Symbol

VSIG10

UniProt

Q8N0Z9

Additional VSIG10 Products

Product Documents for Human VSIG10 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VSIG10 Antibody

For research use only

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