HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) followed by APC-conjugated Goat-anti Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Human VSIG3 ELISA Standard Curve.

Recombinant Human VSIG3 protein was serially diluted 2-fold and captured by Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human VSIG3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human/Mouse VSIG3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4915).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VSIG3 DuoSet ELISA Kit (Catalog # DY9229-05) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human VSIG3 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG3

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Alternate Names

BTIGSF, CXADRL1, IGSF11

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Gene Symbol

IGSF11

UniProt

Q5DX21 (Human)

Additional VSIG3 Products

Product Documents for Human VSIG3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human VSIG3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VSIG3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9229

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all VSIG3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VSIG3 Antibody

Detection of VSIG3 in HEK293 Human Cell Line Transfected with Human VSIG3 and eGFP by Flow Cytometry.