In a functional flow cytometry test, recombinant human VISTA/B7-H5 (20 ng/mL, Catalog # 7126-B7) binds to HEK293 human embryonic kidney cell line transfected with human VSIG8 and eGFP. (A) Binding is completely blocked by 2.5 µg/mL of Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94182) but not by (B) Mouse IgG2B Isotype Control (Catalog # MAB0041). Binding of recombinant human B7-H5/VISTA on cells is detected with anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A).

Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human VSIG8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94182) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human VSIG8 Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

2.5 µg/mL
Sample: Blockade of VISTA/B7-H5 Binding to VSIG8-transfected HEK293 Human Cell Line.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

Q5VU13

Additional VSIG8 Products

Product Documents for Human VSIG8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human VSIG8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VSIG8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB94182

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all VSIG8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VSIG8 Antibody

VISTA/B7-H5 Binding to VSIG8-transfected HEK293 Human Cell Line is Blocked by Human VSIG8 Antibody.