Human IgG Fc APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB110A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Ovine
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 97924
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IgG Fc APC-conjugated Antibody
Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.Applications for Human IgG Fc APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human IgG Fc APC-conjugated Antibody
Product Specific Notices for Human IgG Fc APC-conjugated Antibody
For research use only
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