Human Wnt-7b Antibody

Detection of Human Wnt‑7b by Western Blot.

Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt-7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Wnt-7b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Wnt-7b in Human Breast Cancer

Wnt-7b was detected in immersion fixed paraffin-embedded sections of human breast cancer using Goat Anti-Human Wnt-7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Wnt-7b by Western Blot

Analysis of deltaNp73 in epidermal programming using an induced basal keratinocyte (iKC) model system.(A) Immunoblot of KLF4, p63 alpha, and p73 protein expression in neonatal human dermal fibroblast (HDFn) cells infected with lentivirus encoding deltaNp73 isoforms ( deltaNp73 alpha and deltaNp73 beta) or empty vector control in combination with KLF4 and deltaNp63 alpha. Cells were grown for 3 days and protein was harvested for immunoblot analysis. (B) Bar graphs of RNA expression for the indicated keratinocyte genes in HDFn cells infected in (A). Cells were grown for 3 days and RNA was harvested for qRT-PCR analysis. Expression data are represented as the fold increase relative to control. The mean of three replicates is shown with error bars representing SEM. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. (C) Principal component analysis (PCA) plot of RNA-seq from HDFn cells infected with lentivirus encoding deltaNp73 beta or empty vector control in combination with KLF4 and deltaNp63 alpha. Cells were grown for 6 days and RNA was harvested for RNA-seq analysis. The percentage of variance contributed by each PC is listed in parentheses. (D and E) Tables listing the enriched Genome Ontology (GO) categories and pathways among the top 250 genes contributing to PC1 from (C). (F) Heatmap with the expression of a set of 44 genes that underlie the enrichment of GO categories from (D). Genes are annotated based on known roles in iKC-related processes (gray box) and the presence of a p63/p73 ChIP-seq peak within 50 kb of its TSS in multiple basal cell types (brown box). See also S5 and S6 Figs and S2–S9 Tables. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.