Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Wnt-9a Antibody

Detection of Human Wnt-9a by Western Blot.

Western blot shows lysates of KATO-III human gastric carcinoma cell line and ME-180 human cervical epithelial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human Wnt-9a Monoclonal Antibody (Catalog # MAB3157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Wnt-9a (20 ng) was included. Specific bands for native Wnt-9a were detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Detection of Wnt-9a in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Wnt-9a Monoclonal Antibody (Catalog # MAB3157, filled histogram) or isotype control AA175antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human Wnt-9a Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample:

MCF-7 human breast cancer cell line fixed with paraformaldehye and permeabilized with saponin

Western Blot

5 µg/mL
Sample: KATO-III human gastric carcinoma cell line and ME-180 human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-9a

Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic and breast cancer cells. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.

Long Name

Wingless-type MMTV Integration Site Family, Member 9A

Alternate Names

Wnt-14, Wnt9a

Entrez Gene IDs

7483 (Human); 216795 (Mouse)

Gene Symbol

WNT9A

UniProt

O14904.2

Additional Wnt-9a Products

Product Documents for Human Wnt-9a Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Wnt-9a Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Wnt-9a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3157

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Wnt-9a Antibodies »

Product Specifications

Immunogen

Specificity