Human Wnt-9a Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3157
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Wnt-9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Specificity
Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑9b is observed and less than 1% cross-reactivity with rmWnt-1 and rmWnt-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Wnt-9a Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human Wnt-9a
Sample: Recombinant Human Wnt-9a
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-9a
Long Name
Wingless-type MMTV Integration Site Family, Member 9A
Alternate Names
Wnt-14, Wnt9a
Gene Symbol
WNT9A
UniProt
Additional Wnt-9a Products
Product Documents for Human Wnt-9a Antibody
Product Specific Notices for Human Wnt-9a Antibody
For research use only
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