Human XBP1 (S Isoform) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4257
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 525904
Product Specifications
Immunogen
E. coli-derived recombinant human XBP1S
Ser188-Leu287
Accession # NP_001073007
Ser188-Leu287
Accession # NP_001073007
Specificity
Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human XBP1 (S Isoform) Antibody
Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry.
RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human XBP1 (S Isoform) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: RPMI 8226 human multiple myeloma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: RPMI 8226 human multiple myeloma cell line fixed with paraformaldehyde and permeabilized with saponin
Reviewed Applications
Read 1 review rated 5 using MAB4257 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XBP1
Long Name
X-box Binding Protein 1
Alternate Names
HTF, TREB5, XBP2
Gene Symbol
XBP1
UniProt
Additional XBP1 Products
Product Documents for Human XBP1 (S Isoform) Antibody
Product Specific Notices for Human XBP1 (S Isoform) Antibody
For research use only
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