Detects human XBP1 (S Isoform) (XBP1S) in direct ELISAs. This segment is found only in the XBP1S (spliced) variant. Does not detect XBP1U (unspliced).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human XBP1 (S Isoform) Antibody

Detection of XBP1S in RPMI 8226 Human Cell Line by Flow Cytometry.

RPMI 8226 human multiple myeloma cell line was stained with Mouse Anti-Human XBP1S Monoclonal Antibody (Catalog # MAB4257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human XBP1 (S Isoform) Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: RPMI 8226 human multiple myeloma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB4257 in the following applications:

Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XBP1

X-box binding protein 1 (XBP1; also tax-responsive element-binding protein 5 (TREB5) is a ubiquitously expressed member of the bZIP family of proteins. The spliced form of XBP1 (XBP1S) is a 376 amino acid (aa), 55 kDa transcription factor essential for the unfolded protein response. XBP1S is formed during endoplasmic reticulum stress by IRE-1-mediated splicing that creates a 210 aa substitution for XBP1 aa 161-261. XBP1S contains a basic DNA binding motif (aa 72-94) and a leucine-zipper domain (aa 98-126). Within the region used as an immunogen, human XBP1S shares 96% aa sequence identity with mouse XBP1S.

Long Name

X-box Binding Protein 1

Alternate Names

HTF, TREB5, XBP2

Entrez Gene IDs

7494 (Human); 22433 (Mouse); 289754 (Rat)

Gene Symbol

XBP1

UniProt

NP_001073007

Additional XBP1 Products

Product Documents for Human XBP1 (S Isoform) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human XBP1 (S Isoform) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human XBP1 (S Isoform) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4257

Key Product Details

Species Reactivity

Validated:

Cited:

Applications