Human XIAP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8222
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 117305
Product Specifications
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Met1-Ser497
Accession # P98170
Specificity
Detects human XIAP in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human XIAP Antibody
Human XIAP ELISA Standard Curve.
Recombinant Human XIAP protein was serially diluted 2-fold and captured by Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8222) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human XIAP Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total XIAP DuoSet IC ELISA Kit (Catalog # DYC822-2) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XIAP
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Human XIAP Antibody
Product Specific Notices for Human XIAP Antibody
For research use only
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