Human ZFP90 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7870
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ZFP90
Lys94-Lys207
Accession # Q8TF47
Lys94-Lys207
Accession # Q8TF47
Specificity
Detects human ZFP90 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse ZFP90 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ZFP90 Antibody
Detection of Human ZFP90 by Western Blot.
Western blot shows lysates of IMR-32 human neuroblastoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZFP90 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZFP90 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human ZFP90 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and Bowes human melanoma cell line
Sample: IMR‑32 human neuroblastoma cell line and Bowes human melanoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZFP90
ZFP90 (Zinc Finger Protein 90; also ZFP756 and NK10) is a nuclear 66-72 kDa member of the ZNF505 clade, ZNF91 subfamily, kruppel C2H2-type zinc finger family of proteins. It is widely expressed, and found in cell types such as hepatocytes and cardiac myocytes. Functionally, ZFP90 acts as a transcriptional repressor by interacting with KAP-1/TRIM28/TIF1 beta. This interaction influences histone modifications and heterochromatin protein 1 deposition on DNA. Conversely, it is also known to bind to NRSF, thereby promoting NRSF sensitive gene expression. Human ZFP90 is 636 amino acids (aa) in length. It contains one KRAB domain (aa 14-85) followed by thirteen C2H2-type Zn-finger domains (aa 211-631). Over aa 94-207, human and mouse ZFP90 share 54% aa sequence identity.
Long Name
Zinc Finger Protein 90
Alternate Names
FoxP3-interacting Protein, ZNF756
Gene Symbol
ZFP90
UniProt
Additional ZFP90 Products
Product Documents for Human ZFP90 Antibody
Product Specific Notices for Human ZFP90 Antibody
For research use only
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