ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZnT-8 Monoclonal Antibody (Catalog # MAB7936) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ZNT-8 in PANC-1 Human Cell Line by Flow Cytometry.

PANC-1 human cell line was stained with Mouse Anti-Human ZNT-8 Monoclonal Antibody (Catalog # MAB7936, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human ZnT-8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: PANC-1 human cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using MAB7936 in the following applications:

ELISA (1 Review)
Immunocytochemistry/Immunofluorescence (1 Review)
Microarray (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZnT-8

ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta‑cells and alpha-cells, B cells, and adipocytes and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta-cells, provides a necessary component for proper insulin processing and granule storage. Furthermore, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.

Long Name

Zinc Transporter 8

Alternate Names

SLC30A8, ZnT8

Entrez Gene IDs

169026 (Human); 239436 (Mouse); 299903 (Rat)

Gene Symbol

SLC30A8

UniProt

Q8IWU4 (Human)

Additional ZnT-8 Products

Product Documents for Human ZnT-8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ZnT-8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ZnT-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7936

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ZnT-8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ZnT-8 Antibody

ZnT‑8 in Human Pancreas.