Human ZSCAN21 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8015

R&D Systems, part of Bio-Techne
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AF8015
AF8015-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ZSCAN21 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZSCAN21
Asn139-Asp223
Accession # Q9Y5A6

Specificity

Detects human ZSCAN21 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ZSCAN21 Antibody

Detection of Human ZSCAN21 antibody by Western Blot.

Detection of Human ZSCAN21 by Western Blot.

Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, COLO 205 human colorectal adenocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ZSCAN21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZSCAN21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human ZSCAN21 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Caki‑2 human clear cell carcinoma epithelial cell line, COLO 205 human colorectal adenocarcinoma cell line, and NTera‑2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZSCAN21

ZSCAN21 (Zinc finger and SCAN domain-containing protein 21; also Zfp-38, NY-REN-21, Zipro1 and Ru49) is a nuclear member of the multi-finger SCAN box protein family. It is expressed in select cell types, including cerebellar granule cells, Schwann cells, cerebral cortical and sympathetic neurons, round spermatids, and cells of the hair follicles plus sebaceous glands. It may have multiple functions, one of which is to initiate transcription of neuronal alpha-synuclein, while a second may be to promote granule cell proliferation. Human ZSCAN21 is 473 amino acids (aa) in length. It contains one SCAN (SREZBP, Ctfin51, AW-1/ ZNF174, and Number 18) domain (aa 45-127) that mediates dimerization, followed by seven consecutive C2H2-type zinc-finger regions (aa 277-466). ZSCAN21 is known to form both noncovalent homodimers and heterodimers with SCAND1. There is one potential isoform variant that shows a 188 aa substitution for aa 263-473. Over aa 139-223, human ZSCAN21 shares 54% aa sequence identity with mouse ZSCAN21.

Long Name

Zinc Finger and SCAN Domain Containing 21

Alternate Names

KOX25, NY-REN-21, ZFP383, Zipro1, ZNF38

Entrez Gene IDs

7589 (Human)

Gene Symbol

ZSCAN21

UniProt

Q9Y5A6

Additional ZSCAN21 Products

Product Documents for Human ZSCAN21 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZSCAN21 Antibody

For research use only

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