Hyaluronidase 1/HYAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00201

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Hyaluronidase 1/HYAL1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ENTRTKESCQAIKEY corresponding to internal region according to NP_009296.1, NP_695014.1, NP_695015.1, NP_695017.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1; NP_149349.2; NP_695013.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Hyaluronidase 1/HYAL1 Antibody

Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201]

Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Applications for Hyaluronidase 1/HYAL1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human brain (cerebellum, frontal cortex) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 48.4 kDa band according to isoform 1 (NP_009296.1, NP_149349.2, NP_695013.1), 27.9 kDa band according to isoform 3 (NP_695015.1) and 19.4 kDa band according to isoform 5 (NP_695017.1). The 35 kDa band was successfully blocked by incubation with the immunizing peptide.

Reviewed Applications

Read 1 review rated 3 using NBP1-00201 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hyaluronidase 1/HYAL1

HYAL1 codes for a protein that may have a role in promoting tumor progression and blocking the TGFB1-enhanced cell growth, and is expressed highly in the liver, kidney, and heart, with weaker expression in the lung placenta, and skeletal muscle. There are seven isoforms of HYAL1, with the longest being isoform 1, which is only expressed in bladder and prostate cancer cells and is 435 amino acids long and weighs approximately 48 kDa. Current studies are being done on several diseases and disorders relating to this gene including lung carcinoma, lysosomal storage disease, diabetes mellitus, ovarian cancer, endometrial carcinoma, prostate cancer, and breast cancer. HYAL1 has also been shown to have interactions with GUSB, ARSB, IDUA, FAM107B, and COL2A1 in pathways such as the metabolic, lysosome, and Maroteaux-Lamy syndrome pathways.

Long Name

Hyaluronoglucosaminidase-1

Alternate Names

FUS2, HYAL1, Hyaluronoglucosaminidase 1, LUCA1, NAT6

Entrez Gene IDs

3373 (Human)

Gene Symbol

HYAL1

Additional Hyaluronidase 1/HYAL1 Products

Product Documents for Hyaluronidase 1/HYAL1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hyaluronidase 1/HYAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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