HYI Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81659
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ALGCPRIHLMAGRVPQGADRIAVKAEMEAVFLENLRHAAGVLAQEDLVGLLEPINTRITDPQYFLDTPQQAAAILQKVGRPNLQLQMDIFHWQIMDGNLTGNIREFLPIVGHVQVAQVPGRGEPSSPGELNFPYLFQLLEDEGYKGFVGC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HYI Antibody
Western Blot: HYI Antibody [NBP1-81659]
Western Blot: HYI Antibody [NBP1-81659] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4Immunocytochemistry/ Immunofluorescence: HYI Antibody [NBP1-81659]
Immunocytochemistry/Immunofluorescence: HYI Antibody [NBP1-81659] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.Immunohistochemistry: HYI Antibody [NBP1-81659]
Immunohistochemistry: HYI Antibody [NBP1-81659] - Staining of human ovary shows cytoplasmic positivity in ovarian stroma cells.Applications for HYI Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HYI
Alternate Names
EC 5.3.1.22, Endothelial cell apoptosis protein E-CE1, HT036, hydroxypyruvate isomerase (putative), hydroxypyruvate isomerase homolog, hydroxypyruvate isomerase homolog (E. coli), MGC20767, putative hydroxypyruvate isomerase
Gene Symbol
HYI
Additional HYI Products
Product Documents for HYI Antibody
Product Specific Notices for HYI Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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