ICAM-3/CD50 Antibody (186-2G9) [Janelia Fluor® 646]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34521JF646
Clone 186-2G9 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Janelia Fluor 646
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 186-2G9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ICAM-3/CD50 Antibody (186-2G9) [Janelia Fluor® 646]
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Applications for ICAM-3/CD50 Antibody (186-2G9) [Janelia Fluor® 646]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: ICAM-3/CD50
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for ICAM-3/CD50 Antibody (186-2G9) [Janelia Fluor® 646]
Product Specific Notices for ICAM-3/CD50 Antibody (186-2G9) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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