ICAM-3/CD50 Antibody (SPM505)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32826
![Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) [NBP2-32826]](https://resources.bio-techne.com/images/products/ICAM-3-CD50-Antibody-SPM505-Immunohistochemistry-Paraffin-NBP2-32826-img0001.jpg "Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) [NBP2-32826]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM505
Concentration
0.2 mg/ml
Product Summary for ICAM-3/CD50 Antibody (SPM505)
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34776)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ICAM-3/CD50 Antibody (SPM505)
Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) [NBP2-32826]
Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) [NBP2-32826] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (SPM505).Applications for ICAM-3/CD50 Antibody (SPM505)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ICAM-3/CD50
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Gene Symbol
ICAM3
UniProt
Additional ICAM-3/CD50 Products
Product Documents for ICAM-3/CD50 Antibody (SPM505)
Product Specific Notices for ICAM-3/CD50 Antibody (SPM505)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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