ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44548
![Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted [NBP2-44548]](https://resources.bio-techne.com/images/products/ICAM-3-CD50-Antibody-SPM505-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44548-img0001.jpg "Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted [NBP2-44548]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM505
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted [NBP2-44548]
Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted [NBP2-44548] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (SPM505).Applications for ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ICAM-3/CD50
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
Product Specific Notices for ICAM-3/CD50 Antibody (SPM505) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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