ICAM-5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53815

Novus Biologicals, part of Bio-Techne
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NB100-53815
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ICAM-5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ESPAEGEVFAIQ corresponding to C-Terminus according to NP_003250.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ICAM-5 Antibody

Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815]

Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815]

Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815] - Analysis of ICAM5 precursor in paraffin-embedded Human Brain (Cerebral Cortex) using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ICAM-5 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 200-250 kDa band in lysates of human brain (frontal cortex and hippocampus) and mouse brain after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2 kDa band according to NP_003250.2. The 200-250 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cerebral cortex) shows axon/dendrite staining leaving cell bodies relatively unstained.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICAM-5

ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Long Name

Intercellular Adhesion Molecule 5

Alternate Names

ICAM5, Telencephalin

Entrez Gene IDs

7087 (Human); 15898 (Mouse); 313785 (Rat)

Gene Symbol

ICAM5

UniProt

NP_003250.2 (Human)

Additional ICAM-5 Products

Product Documents for ICAM-5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ICAM-5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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