IDH2 Antibody (5F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00003418-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IDH2 Antibodies »

Product Specifications

Immunogen

IDH2 (NP_002159, 354 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGR

Localization

Mitochondrial

Specificity

IDH2 - isocitrate dehydrogenase 2 (NADP+), mitochondrial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IDH2 Antibody (5F11)

Western Blot: IDH2 Antibody (5F11) [H00003418-M01]

Western Blot: IDH2 Antibody (5F11) [H00003418-M01]

Western Blot: IDH2 Antibody (5F11) [H00003418-M01] - Analysis of IDH2 expression in transfected 293T cell line by IDH2 monoclonal antibody (M01), clone 5F11.Lane 1: IDH2 transfected lysate(50.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: IDH2 Antibody (5F11) [H00003418-M01]

Immunohistochemistry-Paraffin: IDH2 Antibody (5F11) [H00003418-M01]

Immunohistochemistry-Paraffin: IDH2 Antibody (5F11) [H00003418-M01] - Analysis of monoclonal antibody to IDH2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
ELISA: IDH2 Antibody (5F11) [H00003418-M01]

ELISA: IDH2 Antibody (5F11) [H00003418-M01]

ELISA: IDH2 Antibody (5F11) [H00003418-M01] - Detection limit for recombinant GST tagged IDH2 is approximately 0.03ng/ml as a capture antibody.

Applications for IDH2 Antibody (5F11)

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Entrez Gene IDs

3418 (Human)

Gene Symbol

IDH2

OMIM

147650 (Human)

UniProt

NP_002159 (Human)

Additional IDH2 Products

Product Documents for IDH2 Antibody (5F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDH2 Antibody (5F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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