IDH3A Antibody (OTI2F11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02158

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2F11

Concentration

1 mg/ml

View all IDH3A Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDH3A Antibody (OTI2F11)

Western Blot: IDH3A Antibody (2F11) [NBP2-02158] Analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: IDH3A Antibody (OTI2F11) [NBP2-02158]

Immunocytochemistry/ Immunofluorescence: IDH3A Antibody (OTI2F11) [NBP2-02158]

Immunocytochemistry/Immunofluorescence: IDH3A Antibody (2F11) [NBP2-02158] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A.
Immunohistochemistry-Paraffin: IDH3A Antibody (OTI2F11) [NBP2-02158]

Immunohistochemistry-Paraffin: IDH3A Antibody (OTI2F11) [NBP2-02158]

Immunohistochemistry-Paraffin: IDH3A Antibody (2F11) [NBP2-02158] - Staining of paraffin-embedded liver tissue using anti-IDH3A mouse monoclonal antibody.
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Applications for IDH3A Antibody (OTI2F11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDH3A

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

Alternate Names

EC 1.1.1, EC 1.1.1.41, H-IDH alpha, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitrate dehydrogenase 3 (NAD+) alpha, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH

Entrez Gene IDs

3419 (Human)

Gene Symbol

IDH3A

Additional IDH3A Products

Product Documents for IDH3A Antibody (OTI2F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDH3A Antibody (OTI2F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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