IDO2 Antibody (1HC)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21641
![Western Blot: IDO2 Antibody (1HC) [NBP2-21641]](https://resources.bio-techne.com/images/products/IDO2-Antibody-1HC-Western-Blot-NBP2-21641-img0002.jpg "Western Blot: IDO2 Antibody (1HC) [NBP2-21641]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1HC
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
IDO2 Antibody (1HC) was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development. (Uniprot: Q8R0V5)
Reactivity Notes
Cross-reactivity with IDO2 from other sources has not been determined
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
This antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for IDO2 Antibody (1HC)
Western Blot: IDO2 Antibody (1HC) [NBP2-21641]
Western Blot: IDO2 Antibody (1HC) [NBP2-21641] - Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK.Lane 3: mouse IDO-1 transgene expressed in 293HEK. IDO2 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band: IDO2 splice variants and isoforms.Applications for IDO2 Antibody (1HC)
Application
Recommended Usage
ELISA
1:5000-1:50000
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Western Blot
1:500-1:1500
Application Notes
This product has been tested in western blot and is suitable for use in ELISA and IF. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: IDO2
Long Name
Indoleamine 2,3-dioxygenase 2
Alternate Names
Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2
Entrez Gene IDs
209176 (Mouse)
Gene Symbol
IDO2
UniProt
Additional IDO2 Products
Product Documents for IDO2 Antibody (1HC)
Product Specific Notices for IDO2 Antibody (1HC)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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