IDO2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45209
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RDKTLESILHPR corresponding to C-Terminus according to NP_919270.2.
Epitope
C-Terminus
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for IDO2 Antibody
Western Blot: IDO2 Antibody [NBP1-45209]
Western Blot: IDO2 Antibody [NBP1-45209] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Frozen: IDO2 Antibody [NBP1-45209]
Immunohistochemistry-Frozen: IDO2 Antibody [NBP1-45209] - Pieces of the skin sample used to manufacture the fibroblast and keratinocyte cell banks were frozen and embedded for cryosectioning. Skin sections were stained for the immunodetection of IDO-2. Isotypic controls for IDO-2 staining are indicated in the publication. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0070408), licensed under a CC-BY license.Applications for IDO2 Antibody
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10 - 1:500
Peptide ELISA
Detection limit 1:64000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human kidney lysates (calculated MW of 47.07 kDa band according to NP_919270.2). IHC-F usage is reported in scientific literature (PMID: 23894651).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IDO2
Long Name
Indoleamine 2,3-dioxygenase 2
Alternate Names
Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2
Entrez Gene IDs
169355 (Human)
Gene Symbol
IDO2
UniProt
Additional IDO2 Products
Product Documents for IDO2 Antibody
Product Specific Notices for IDO2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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