IER3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76802

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IER3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1. The immunogen is located within amino acids 40 - 90 of IEX-1. Amino Acid Squence: APAGRPSASRGHRKRSRRVLYPR

Reactivity Notes

Rat reactivity reported in (PMID: 22417305).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IER3 Antibody - BSA Free

Western Blot: IER3 AntibodyBSA Free [NBP1-76802]

Western Blot: IER3 AntibodyBSA Free [NBP1-76802]

Western Blot: IER3 Antibody [NBP1-76802] - Analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 ug/mL..
Immunocytochemistry/ Immunofluorescence: IER3 Antibody - BSA Free [NBP1-76802]

Immunocytochemistry/ Immunofluorescence: IER3 Antibody - BSA Free [NBP1-76802]

Immunocytochemistry/Immunofluorescence: IER3 Antibody [NBP1-76802] - Human Brain cells with IEX-1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: IER3 Antibody - BSA Free [NBP1-76802]

Immunohistochemistry-Paraffin: IER3 Antibody - BSA Free [NBP1-76802]

Immunohistochemistry-Paraffin: IER3 Antibody [NBP1-76802] - Human liver tissue with IEX-1 antibody at 2.5 ug/mL
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Applications for IER3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-76802 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IER3

IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-alpha. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappaB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.

Alternate Names

DIF-2, DIF2GLY96, Differentiation-dependent gene 2 protein, expressed in pancreatic carcinoma, gly96, mouse, homolog of, IEX-1, IEX1death protein, IEX-1L, Immediate early protein GLY96, immediate early response 3, Immediate early response 3 protein, immediately early gene X-1, PRG1PACAP-responsive gene 1 protein, Protein DIF-2, Protein PRG1, radiation-inducible immediate-early gene IEX-1

Entrez Gene IDs

8870 (Human); 15937 (Mouse)

Gene Symbol

IER3

UniProt

P46695 (Human)

Additional IER3 Products

Product Documents for IER3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IER3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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