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IFN-alpha 1 Antibody (2-48)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07232

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07232-100ug
NBP3-07232-20ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2-48

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Purified recombinant human IFN-alpha 1 (Uniprot: P01562)

Localization

Cytoplasmic & Extracellular (Secreted)

Specificity

This monoclonal antibody is specific for human interferon alpha 1 and does not cross react with human interferon alpha 2. Interferons are widely used therapeutic agents because of their anti-tumor and anti-viral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon- Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is influenced by phosphorylation of Serine 535 and 539 in the IFNAR1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08231)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for IFN-alpha 1 Antibody (2-48)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IFN-alpha 1

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha 1

Alternate Names

IFNA1, IFNalpha 1

Gene Symbol

IFNA1

UniProt

Additional IFN-alpha 1 Products

Product Documents for IFN-alpha 1 Antibody (2-48)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IFN-alpha 1 Antibody (2-48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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