Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IFN-alpha/beta R1 Antibodies »

Product Summary for IFN-alpha/beta R1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 507 to 557 of human Interferon (alpha, beta and omega) Receptor 1 using the numbering given in entry NP_000620.2 (GeneID 3454).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFN-alpha/beta R1 Antibody

Western Blot: IFN-alpha/beta R1 Antibody [NBP2-32196]

Western Blot: IFN-alpha/beta R1 Antibody [NBP2-32196]

Western Blot: IFN-alpha/beta R1 Antibody [NBP2-32196] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-IFNAR1 antibody NBP2-32196 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: IFN-alpha/beta R1 Antibody [NBP2-32196]

Immunoprecipitation: IFN-alpha/beta R1 Antibody [NBP2-32196]

Immunoprecipitation: IFN-alpha/beta R1 Antibody [NBP2-32196] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IFNAR1 antibody NBP2-32196 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated IFNAR1, NBP2-32196 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for IFN-alpha/beta R1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IFN-alpha/beta R1

Interferon alpha receptor 1 (IFN-alpha R1) is a class II cytokine receptor which belongs to type I human interferons (IFNs) family. IFNs plays a role in antiviral, antiproliferative, immunomodulatory, antitumor, and antiparasitic activities by inducing transcription of IFN-stimuated genes (ISGs) through activation of the Jak-STAT pathway. Specifically, IFN- R1 is a 135-kda signal transducing subunit of the IFN alpha/beta receptor complex (IFN-alpha R) (1). IFN-alpha R1 is phospholyated through PTK- and PKC-mediated phosphorylation, which initiates docking of STAT proteins to IFNs. This leads to activation of STAT proteins by PTK-mediated phosphorylation. The mechanism behind the amplification of IFN-alpha R complex mediated transmembrane signaling has been linked to tyrosine phosphorylation on IFN-alpha R1 (2).

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Gene Symbol

IFNAR1

UniProt

NP_000620.2 (Human)

Additional IFN-alpha/beta R1 Products

Product Documents for IFN-alpha/beta R1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IFN-alpha/beta R1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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