Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # IFNG/466

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IFN-gamma Antibodies »

Product Summary for IFN-gamma Antibody (IFNG/466) - Azide and BSA Free

Immunogen

Purified recombinant human full-length IFN-gamma protein (Uniprot: P01579)

Localization

Cytoplasmic & Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53332).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IFN-gamma Antibody (IFNG/466) - Azide and BSA Free

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)
Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)
Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394]

Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) - Azide and BSA Free [NBP2-54394] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with IFN-gamma Mouse Monoclonal Antibody (IFNG/466).

Applications for IFN-gamma Antibody (IFNG/466) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IFN-gamma

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for IFN-gamma Antibody (IFNG/466) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IFN-gamma Antibody (IFNG/466) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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