IFN-gamma R1/CD119 Antibody (2B9Q1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15421

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15421-100ul
NBP3-15421-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2B9Q1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IFN-gamma R1/CD119 Antibodies »

Product Summary for IFN-gamma R1/CD119 Antibody (2B9Q1)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFN-gamma R1/CD119 (NP_000407.1). IKKINPLKEKSIILPKSLISVVRSATLETKPESKYVSLITSYQPFSLEKEVVCEEPLSPATVPGMHTEDNPGKVEHTEELSSITEVVTTEENIPDVVPGSHLTPIERESSSPLSSNQSEPGSIALNSYHSRNCSESDHSRNGFDTDSSCLESHSSLSDSEFPPNNKGEIKTEGQELITVIKAPTSFGYDKPHVLVDLLVDDSGKESLIGYRPTEDSKEFS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFN-gamma R1/CD119 Antibody (2B9Q1)

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421]

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421]

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421] - Western blot analysis of extracts of various cell lines, using IFN-gamma R1/CD119 Rabbit mAb (NBP3-15421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421]

Immunocytochemistry/ Immunofluorescence: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421]

Immunocytochemistry/Immunofluorescence: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421] - Immunofluorescence analysis of THP-1 cells using IFN-gamma R1/CD119 Rabbit mAb (NBP3-15421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for IFN-gamma R1/CD119 Antibody (2B9Q1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFN-gamma R1/CD119

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Gene Symbol

IFNGR1

Additional IFN-gamma R1/CD119 Products

Product Documents for IFN-gamma R1/CD119 Antibody (2B9Q1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IFN-gamma R1/CD119 Antibody (2B9Q1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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