IgE Antibody (BE5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-470

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BE5

Format

BSA Free

Concentration

1 mg/ml

View all IgE Antibodies »

Product Specifications

Immunogen

Purified human IgE.

Specificity

The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IgE Antibody (BE5) - BSA Free

Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) [NB500-470] - Staining of IgE in human peripheral blood with anti-IgE (BE5) PE-CyTM5.
Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) [NB500-470] - Surface staining of IgE in human peripheral blood lymphocytes with anti-IgE (BE5) purified, GAM-APC.
Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) - BSA Free [NB500-470]

Flow Cytometry: IgE Antibody (BE5) [NB500-470] - Analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Fig. 1B - staining of IgE stimulated sample
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Applications for IgE Antibody (BE5) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgE

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for IgE Antibody (BE5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgE Antibody (BE5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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