Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4B12

Concentration

1 mg/ml

View all IGF-I/IGF-1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-151 of human IGF1(NP_001104753) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGF-I/IGF-1 Antibody (OTI4B12)

Western Blot: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Western Blot: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Western Blot: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF-1.
Immunohistochemistry: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Immunohistochemistry: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Immunohistochemistry: IGF-I/IGF-1 Antibody (OTI4B12) [NBP2-46037]

Immunohistochemistry: IGF-I Antibody (4B12) [NBP2-46037] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for IGF-I/IGF-1 Antibody (OTI4B12)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGF-I/IGF-1

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody (OTI4B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IGF-I/IGF-1 Antibody (OTI4B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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