IGF-II/IGF2 Antibody (8H1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37462
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8H1
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human IGF-II/IGF2 (AA: 25-180) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IGF-II/IGF2 Antibody (8H1) - BSA Free
Western Blot: IGF-II/IGF2 Antibody (8H1)BSA Free [NBP2-37462]
Western Blot: IGF-II Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: IGF-II/IGF2 Antibody (8H1) - BSA Free [NBP2-37462]
Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HeLa cells using IGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) - BSA Free [NBP2-37462]
Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of paraffin-embedded bladder cancer tissues using IGF-II/IGF2 (8H1)mouse mAb with DAB staining.Applications for IGF-II/IGF2 Antibody (8H1) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGF-II/IGF2
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for IGF-II/IGF2 Antibody (8H1) - BSA Free
Product Specific Notices for IGF-II/IGF2 Antibody (8H1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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