IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09145

Clone MEM-240 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP3-09145
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-240

Format

BSA Free

Concentration

1 mg/ml

View all IGF-II R/IGF2R Antibodies »

Product Specifications

Immunogen

Recombinant Vaccinia virus encoding IGF-II R/IGF2R.

Specificity

The antibody MEM-238 recognizes an extracellular epitope between amino acids 698-1262 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) - BSA Free [NBP3-09145]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.

Applications for IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-6 ug/ml
Application Notes
Flow cytometry: Extracellular and intracellular staining. Recommended dilution: 2-6 ug/ml. Western blotting: non-reducing conditions. Recommended dilution: 1-2 ug/ml.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-II R/IGF2R

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Gene Symbol

IGF2R

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IGF-II R/IGF2R Antibody (MEM-240) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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