IGFBP-1 Antibody (JE35-39)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32460
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-39
Concentration
1 mg/ml
Product Summary for IGFBP-1 Antibody (JE35-39)
Immunogen
Recombinant protein within human IGFBP-1 aa 1-259/259. (Uniprot: P08833)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IGFBP-1 Antibody (JE35-39)
Western Blot: IGFBP-1 Antibody (JE35-39) [NBP3-32460]
Western Blot: IGFBP-1 Antibody (JE35-39) [NBP3-32460] - Western blot analysis of IGFBP-1 on different lysates with Rabbit anti-IGFBP-1 antibody (NBP3-32460) at 1/1,000 dilution.Lane 1: Human placenta tissue lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 28 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for IGFBP-1 Antibody (JE35-39)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGFBP-1
Long Name
Insulin-like Growth Factor Binding Protein 1
Alternate Names
IGFBP1
Gene Symbol
IGFBP1
Additional IGFBP-1 Products
Product Documents for IGFBP-1 Antibody (JE35-39)
Product Specific Notices for IGFBP-1 Antibody (JE35-39)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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