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IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12364

Novus Biologicals, part of Bio-Techne
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NBP2-12364-0.025mg
NBP2-12364

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0342-6U23

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant IGFBP-3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Applications for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-200

Immunohistochemistry-Paraffin

1:20-1:200

Western Blot

1:500-1:2000
Application Notes
This IGFBP3 antibody (clone MM0342-6U23) is useful for Western Blot and Immunohistochemistry-Paraffin applications. In its IHC-P validation, the antigen retrieval was performed by incubation of sections with Proteinase K (20ug/ml in PBS) for 15min at room temperature (Note: the length of PK exposure should be optimized by the user as it may depend upon the variation of fixatives and length of fixation applied to a particular specimen).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGFBP-3

IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Insulin-like Growth Factor Binding Protein 3

Alternate Names

IGFBP3

Gene Symbol

IGFBP3

UniProt

Additional IGFBP-3 Products

Product Documents for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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