IGFBP-3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00203
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RYKVDYESQSTDTQN corresponding to internal region according to NP_000589.2.
Specificity
This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for IGFBP-3 Antibody
![Western Blot: IGFBP-3 Antibody [NBP1-00203]](https://resources.bio-techne.com/images/products/IGFBP-3-Antibody-Western-Blot-NBP1-00203-img0002.jpg "Western Blot: IGFBP-3 Antibody [NBP1-00203]")
Western Blot: IGFBP-3 Antibody [NBP1-00203]
Western Blot: IGFBP-3 Antibody [NBP1-00203] - (0.02ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for IGFBP-3 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
0.02 - 0.06 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human breast cancer lysates (calculated MW of 31.7 kDa band according to NP_001013416.1 and 32.2 kDa band according to NP_000589.2).
An additional band of 50 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IGFBP-3
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
UniProt
Additional IGFBP-3 Products
Product Documents for IGFBP-3 Antibody
Product Specific Notices for IGFBP-3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...