IGFBP-3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16933
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human IGFBP-3. The exact sequence is proprietary.
Reactivity Notes
Bovine, Pig, Rhesus Monkey (80%).
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IGFBP-3 Antibody
![Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933]](https://resources.bio-techne.com/images/products/IGFBP-3-Antibody-Immunohistochemistry-Frozen-NBP2-16933-img0002.jpg "Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933]")
Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933]
Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933] - Sample: Frozen sectioned E13.5 Rat brain. Green: IGFBP3 protein stained by IGFBP3 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.![Western Blot: IGFBP-3 Antibody [NBP2-16933]](https://resources.bio-techne.com/images/products/IGFBP-3-Antibody-Western-Blot-NBP2-16933-img0003.jpg "Western Blot: IGFBP-3 Antibody [NBP2-16933]")
Western Blot: IGFBP-3 Antibody [NBP2-16933]
Western Blot: IGFBP-3 Antibody [NBP2-16933] - Mouse plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: IGFBP-3 Antibody [NBP2-16933] -
Western Blot: IGFBP-3 Antibody [NBP2-16933] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP-3 antibody [N2C3] (NBP2-16933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for IGFBP-3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: IGFBP-3
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
UniProt
Additional IGFBP-3 Products
Product Documents for IGFBP-3 Antibody
Product Specific Notices for IGFBP-3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.