IgG Antibody (IG266) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34648

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # IG266

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all IgG Antibodies »

Product Specifications

Immunogen

Purified human IgG

Localization

Cytoplasm, Cell Surface and Secreted

Specificity

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34250).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IgG Antibody (IG266) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Immunocytochemistry/Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).
Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]

Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for IgG Antibody (IG266) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID:10.1371/journal.pntd.0009390)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for IgG Antibody (IG266) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IgG Antibody (IG266) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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