IgG Antibody (IG266) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34648
![Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]](https://resources.bio-techne.com/images/products/IgG-Antibody-IG266-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34648-img0002.jpg "Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # IG266
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human IgG
Localization
Cytoplasm, Cell Surface and Secreted
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34250).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for IgG Antibody (IG266) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]
Immunocytochemistry/Immunofluorescence: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.![Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]](https://resources.bio-techne.com/images/products/IgG-Antibody-IG266-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34648-img0003.jpg "Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]")
Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]
Immunohistochemistry-Paraffin: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).![Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]](https://resources.bio-techne.com/images/products/IgG-Antibody-IG266-Azide-and-BSA-Free-Protein-Array-NBP2-34648-img0001.jpg "Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]")
Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648]
Protein Array: IgG Antibody (IG266) - Azide and BSA Free [NBP2-34648] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for IgG Antibody (IG266) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID:10.1371/journal.pntd.0009390)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for IgG Antibody (IG266) - Azide and BSA Free
Product Specific Notices for IgG Antibody (IG266) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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