IgG2a Antibody (RM219) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25952
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM219
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for IgG2a Antibody (RM219) - Azide and BSA Free
Immunogen
Mouse IgG2a
Specificity
This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG2a Antibody (RM219) - Azide and BSA Free
Western Blot: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -
Western Blot: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using Clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).
ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -
ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -
ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Applications for IgG2a Antibody (RM219) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.005 ug/ml - 0.2 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for IgG2a Antibody (RM219) - Azide and BSA Free
Product Specific Notices for IgG2a Antibody (RM219) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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