IgG4 Antibody (RM120) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62126B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA Capture, Sandwich ELISA Detection, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM120
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide corresponding to the hinge region of Human IgG4
Specificity
This antibody reacts to the heavy chain of human IgG4, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG4 Antibody (RM120) [Biotin]
Western Blot: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Western Blot of human, mouse, rat, and goat IgG shows NBP2-62126B reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
Immunohistochemistry: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG4 antibody NBP2-62126B.
ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - A titer ELISA using NBP2-62126B. The plate was coated with different amounts of human IgG4. A serial dilution of NBP2-62126B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for IgG4 Antibody (RM120) [Biotin]
Application
Recommended Usage
Flow Cytometry
1ug/ml - 4ug/ml
Immunocytochemistry
1ug/mL - 10ug/mL
Immunocytochemistry/ Immunofluorescence
1 - 10 ug/ml
Immunohistochemistry
1ug/mL - 10ug/mL
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS with 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG4
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G4
Alternate Names
Immunoglobulin G, Immunoglobulin G4, ImmunoglobulinG
Additional IgG4 Products
Product Documents for IgG4 Antibody (RM120) [Biotin]
Product Specific Notices for IgG4 Antibody (RM120) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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