IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34601
![Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free [NBP2-34601]](https://resources.bio-techne.com/images/products/IgM-Antibody-DA4-4-same-as-SA-DA4-or-HB57-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34601-img0001.jpg "Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free [NBP2-34601]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DA4-4 (same as SA-DA4 or HB57)
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
Immunogen
Heavy chain of human IgM
Localization
Cytoplasm, Cell Surface and Secreted
Specificity
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34252).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free [NBP2-34601]
Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free [NBP2-34601] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (DA4-4)Applications for IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: IgM
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbol
IGHM
Additional IgM Products
Product Documents for IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
Product Specific Notices for IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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