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IgM Antibody (II/41) [PE/Cy5]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43068

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry

Label

PE/Cy5 (Excitation = 480;565;650 nm, Emission = 670 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # II/41

Concentration

0.2 mg/ml

Product Summary for IgM Antibody (II/41) [PE/Cy5]

Immunogen

The immunogen for this antibody was IgM.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for IgM Antibody (II/41) [PE/Cy5]

Flow Cytometry: Mouse IgM Antibody (II/41) [PE/Cy5] [NBP1-43068] - Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 ug of Rat IgG2a Isotype Control PE-Cy5 (left) or 0.06 ug of Anti-Mouse IgM PE-Cy5 (right). Total viable cells were used for analysis.

Applications for IgM Antibody (II/41) [PE/Cy5]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This II/41 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgM

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (II/41) [PE/Cy5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IgM Antibody (II/41) [PE/Cy5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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