IgM Antibody (SPM557) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34422

Novus Biologicals, part of Bio-Techne
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NBP2-34422-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM557

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all IgM Antibodies »

Product Specifications

Immunogen

Recombinant heavy chain of human IgM

Localization

Cytoplasm, Cell Surface and Secreted

Specificity

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34375).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IgM Antibody (SPM557) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422]

Immunocytochemistry/ Immunofluorescence: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422]

Immunocytochemistry/Immunofluorescence: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422] - Analysis of LM7 cells using DyLight 550 conjugated anti-IgM antibody. DAPI Solution (blue; cat.# NBP2-31156) was used as a nuclear counterstain. Image from verified customer review.
Immunohistochemistry-Paraffin: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422]

Immunohistochemistry-Paraffin: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422]

Immunohistochemistry-Paraffin: IgM Antibody (SPM557) - Azide and BSA Free [NBP2-34422] - Formalin-fixed, paraffin-embedded human tonsil stained with igM Monoclonal Antibody (SPM557)

Applications for IgM Antibody (SPM557) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-34422 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (SPM557) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IgM Antibody (SPM557) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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