IGSF4A/SynCAM1/CADM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-186

Novus Biologicals, part of Bio-Techne
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NB300-186
NB300-186SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all IGSF4A/SynCAM1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the C-terminal region of rat SynCAM/CADM1.

Localization

Growth cone

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGSF4A/SynCAM1/CADM1 Antibody

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY CADM1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SynCAM/CADM1.
Immunocytochemistry/ Immunofluorescence: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Immunocytochemistry/ Immunofluorescence: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Immunocytochemistry/Immunofluorescence: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - SynCAM/CADM1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186]

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.
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Applications for IGSF4A/SynCAM1/CADM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Simple Western

1:1000

Western Blot

1:1000
Application Notes
This SynCAM/CADM1 antibody can be used for Western blot and Immunocytochemistry/Immunofluorescence. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. Heterologous cell lysates give a single band around 107kDa. In ICC/IF, membrane staining was observed in SH-SY-5Y cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGSF4A/SynCAM1

CADM1 (cell adhesion molecule 1; SynCAM1) is an intercellular cell adhesion receptor which belongs to immunoglobulin superfamily and is expressed by diverse cell types which includes lung alveolar cells, biliary epithelial cells, pancreatic islet cells, ameloblasts, neurons, and mast cells. Human CADM1 was originally discovered as a tumour suppressor of lung cancer 1, and modulation of its expression, SNPs or mutations in CADM1 are now known to implicate in several diseases including cancer, autism spectrum disorder and venous thrombosis. The expression of CADM1 is significantly reduced in neuroblastoma and cancers of the lung, oesophagus and skin; wherein it is inversely correlated with carcinogenic progression. Its expression is reduced in endothelial cells in venous thrombosis, however, it gets upregulated in adult T-cell leukaemia/lymphoma as well as acute T-cell leukaemia wherein it facilitates tissue infiltration. CADM1 is involved in adhesion via homophilic and heterophilic interactions with other cell's counter-receptors that belongs to same family (CADM1 itself, CADM2, CADM3, nectin3 and CRTAM). CADM1 expression is also increased in neuro-developmental disorder Rett syndrome.

Long Name

Immunoglobulin Superfamily, Member 4

Alternate Names

BL2, Cadm1, Necl-2, RA175, ST17, SynCAM1, TSLC1

Entrez Gene IDs

23705 (Human); 363058 (Rat)

Gene Symbol

CADM1

UniProt

Q9BY67

Additional IGSF4A/SynCAM1 Products

Product Documents for IGSF4A/SynCAM1/CADM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IGSF4A/SynCAM1/CADM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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