Ikaros/IKZF1 Antibody (4S10I3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16223

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16223-100ul
NBP3-16223-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4S10I3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ikaros Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros/IKZF1 (Q13422). AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ikaros/IKZF1 Antibody (4S10I3)

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]

Immunocytochemistry/Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Immunofluorescence analysis of Jurkat cells using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros/IKZF1 antibody (NBP3-16223) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Ikaros/IKZF1 Antibody (4S10I3)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:20 - 1:200

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ikaros

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Long Name

DNA-binding protein Ikaros

Alternate Names

IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1

Gene Symbol

IKZF1

Additional Ikaros Products

Product Documents for Ikaros/IKZF1 Antibody (4S10I3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ikaros/IKZF1 Antibody (4S10I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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