Ikaros/IKZF1 Antibody (4S10I3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16223
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4S10I3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Ikaros/IKZF1 Antibody (4S10I3)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros/IKZF1 (Q13422). AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ikaros/IKZF1 Antibody (4S10I3)
![Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]](https://resources.bio-techne.com/images/products/Ikaros-IKZF1-Antibody-4S10I3-Western-Blot-NBP3-16223-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]")
Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]
Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]](https://resources.bio-techne.com/images/products/Ikaros-IKZF1-Antibody-4S10I3-Immunocytochemistry-Immunofluorescence-NBP3-16223-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]")
Immunocytochemistry/ Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223]
Immunocytochemistry/Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Immunofluorescence analysis of Jurkat cells using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros/IKZF1 antibody (NBP3-16223) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Applications for Ikaros/IKZF1 Antibody (4S10I3)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:20 - 1:200
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ikaros
Long Name
DNA-binding protein Ikaros
Alternate Names
IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1
Gene Symbol
IKZF1
Additional Ikaros Products
Product Documents for Ikaros/IKZF1 Antibody (4S10I3)
Product Specific Notices for Ikaros/IKZF1 Antibody (4S10I3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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