Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all IKBKAP Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1150 and 1200 of human Inhibitor of Kappa Light Polypeptide Gene Enhancer in B-cells, Kiinase Complex-Associated Protein using the numbering given in entry NP_003631.2 (GeneID 8518).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKBKAP Antibody

Western Blot: IKBKAP Antibody [NBP1-71918]

Western Blot: IKBKAP Antibody [NBP1-71918]

Western Blot: IKBKAP Antibody [NBP1-71918] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-IKAP antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IKAP was also immunoprecipitated by rabbit anti-IKAP antibody NBP1-71919, which recognizes a downstream epitope.

Applications for IKBKAP Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IKBKAP

The transcription factor NF-kappa B coordinates the activation of numerous genes in response to pathogens and pro-inflammatory cytokines, and is, therefore, vital in the development of acute and chronic inflammatory diseases. NF-kappa B is activated by cytokine-activated IKB kinases (IKKs); IKK-alpha and IKK-beta isozymes are found in large complexes. Theses large, interleukin-1-inducible IKK complexes have been found to contain a new protein, termed IKK-complex-associated protein (IKAP), which can bind NIK and IKKs and assemble them into an active kinase complex. IKAP is a scaffold protein and a regulator for 3 different kinases involved in pro-inflammatory cytokine signaling.

Alternate Names

DYS, elongator complex protein 1, ELP1DKFZp781H1425, FD, FLJ12497, IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII), IkappaB kinase complex-associated protein, IKI3, IKK complex-associated protein, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein, p150, TOT1

Gene Symbol

ELP1

UniProt

NP_003631.2 (Human)

Additional IKBKAP Products

Product Documents for IKBKAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IKBKAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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