Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IKBKAP Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP. Amino Acid Squence: PPKINRRTQWKLSLLD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (88%)

Specificity

At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105, 145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IKBKAP Antibody - BSA Free

Western Blot: IKBKAP AntibodyBSA Free [NBP1-76788]

Western Blot: IKBKAP AntibodyBSA Free [NBP1-76788]

Western Blot: IKBKAP Antibody [NBP1-76788] - A-20 cell lysate with IKBKAP antibody at in (A) 0.5, and (B) 1 u/mL.

Immunocytochemistry/Immunofluorescence: IKBKAP Antibody [NBP1-76788] -

Immunocytochemistry/Immunofluorescence: IKBKAP Antibody [NBP1-76788] - A-20 cells with IKBKAP antibody at 1 ug/ml.
IKBKAP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IKBKAP Antibody - BSA Free [NBP1-76788] -

Immunocytochemistry/ Immunofluorescence: IKBKAP Antibody - BSA Free [NBP1-76788] - Immunofluorescence of IKBKAP in A20 cells with IKBKAP antibody at 20 ug/mL.

Applications for IKBKAP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1-20 ug/mL

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKBKAP

IKAP was initially identified as a scaffold protein of the IkappaB kinase complex that could bind to IKKalpha, IKKbeta, NF-kappaB, and the NF-kappaB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.

Alternate Names

DYS, elongator complex protein 1, ELP1DKFZp781H1425, FD, FLJ12497, IKAPdysautonomia (Riley-Day syndrome, hereditary sensory autonomic neuropathy typeIII), IkappaB kinase complex-associated protein, IKI3, IKK complex-associated protein, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinasecomplex-associated protein, p150, TOT1

Gene Symbol

ELP1

UniProt

AAC64258 (Human)

Additional IKBKAP Products

Product Documents for IKBKAP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IKBKAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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